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10.1186/s12987-015-0007-9 202 WimmerI.TietzS.NishiharaH.DeutschU.SallustoF.GosseletF.et al

Interestingly, exogenous HGF did not promote spherogenicity under these culture conditions, however, profound induction of sphere formation was seen when EGF and FGF were omitted from the medium in ZH-161 and ZH-305 except T-269

Impurities in synthetic peptides can confound experimental results, making third-party Certificate of Analysis (CoA) documentation a prerequisite for reliable research use